This paper explores the theological significance of the creation of light and darkness in Genesis 1:2-3, emphasizing these elements’ contrasting roles in biblical cosmology. While light is depicted as an inherent attribute of God, symbolizing life, goodness, and creation, darkness is understood as a non-world—a state of cosmic disorder and potential non-existence. The creation narrative, which separates light from darkness, highlights the essential nature of this contrast in defining divine goodness and the world’s order. Drawing on intertextual references from Isaiah, Job, Psalms, and the New Testament, the paper demonstrates that light is not merely a physical phenomenon but a divine manifestation, essential for life and renewal. By examining Isaiah 45:7 and its allusions to Genesis, the paper argues that darkness, though part of the created order, becomes evil only through human and demonic choice, particularly through the rejection of God’s light. This theological framework challenges simplistic notions of dualism, suggesting that the distinction between light and darkness is not merely moral but ontological, with evil arising when humanity aligns itself with darkness, thus disrupting the created order. This analysis also has implications for understanding biblical perspectives on moral and existential separations, including the characterization of same-sex relations as an abomination (tôʕēvāʰ), grounding such discussions in the theological context of creation and divine order.